pLKO.3 Thy1.1
(Plasmid #14749)

• Purpose: (Empty Backbone)
• Depositing Labs: Christophe Benoist, Diane Mathis
• Publication: pLKO variants (unpublished)

Backbone

• Vector backbone: pLKO.3 Thy1.1
• Backbone size (bp): 8000
• Vector type: Mammalian Expression, Lentiviral, RNAi
• Selectable markers: Thy1.1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: PacI (not destroyed)
• 5′ sequencing primer: LKO.1 5'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Derived from the pLKO.1 plasmid from The RNAi Consortium (TRC)
• Reference:
  • Addgene's pLKO.1 protocol
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A variant of pLKO.1 containing an Thy1.1 marker rather than puromycin.

pLKO.3 Thy 1.1 was cloned by excising PAC-GFP from pLKO.3pgw and replacing it with Thy1.1 that was PCR'd from another vector. Cloned into the BamHI and EcoRV sites of pLKO.3pgw.

See http://citdh.org/Lentivectors.html

for more information on pLKO.3pgw.