pLKO.3G Notes
Addgene Diagnostic Digest
The gel depicts the plasmid cut with EcoRI-HF, PacI and EcoRI-HF + KpnI-HF as well as the NEB 1kb ladder.
We see the expected pattern.
Addgene Diagnostic Digest
This gel depicts the NEB 1kb ladder and a digestion of the plasmid pLKO.3G with the following enzymes: BamHI-HF and KpnI-HF.
We expected the following band sizes for this digest-
BamHI-HF: ~8.1kb
KpnI-HF: ~8.1kb
We observe the expected pattern.
