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pCIneo-lambdaN-HA-HsSmg7-G100E-siRNAres-shRNAres_S
(Plasmid #147461)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 147461 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI-neo
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HsSmg7-G100E-siRNAres-shRNAres
  • Species
    H. sapiens (human)
  • Mutation
    three silent mutation G216A, T1185C and A1521G and two non silent mutations Q651R, V854I compared to the sequence given by NCBI: NM_201568.1 isoform2
  • Entrez Gene
    SMG7 (a.k.a. C1orf16, EST1C, SGA56M)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCIneo-lambdaN-HA-HsSmg7-G100E-siRNAres-shRNAres_S was a gift from Elisa Izaurralde (Addgene plasmid # 147461 ; http://n2t.net/addgene:147461 ; RRID:Addgene_147461)

  • For your References section:

    An unusual arrangement of two 14-3-3-like domains in the SMG5-SMG7 heterodimer is required for efficient nonsense-mediated mRNA decay. Jonas S, Weichenrieder O, Izaurralde E. Genes Dev. 2013 Jan 15;27(2):211-25. doi: 10.1101/gad.206672.112. 10.1101/gad.206672.112 PubMed 23348841
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