pcDNA3.1-K20
(Plasmid
#14743)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14743 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5500
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKeratin 20
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Alt nameKRT20
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SpeciesH. sapiens (human)
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Entrez GeneKRT20 (a.k.a. CD20, CK-20, CK20, K20, KRT21)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cloning information may not be accurate. The plasmid was used for transfection by the Omary lab. Also see Zhou et al JBC 2006 281:16453.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-K20 was a gift from M Bishr Omary (Addgene plasmid # 14743 ; http://n2t.net/addgene:14743 ; RRID:Addgene_14743) -
For your References section:
Keratin 20 helps maintain intermediate filament organization in intestinal epithelia. Zhou Q, Toivola DM, Feng N, Greenberg HB, Franke WW, Omary MB. Mol Biol Cell. 2003 Jul . 14(7):2959-71. 10.1091/mbc.e03-02-0059 PubMed 12857878