human K8
(Plasmid
#14742)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14742 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMRB101
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKeratin 8
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Alt nameKRT8
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SpeciesH. sapiens (human)
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Entrez GeneKRT8 (a.k.a. CARD2, CK-8, CK8, CYK8, K2C8, K8, KO)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (destroyed during cloning)
- 5′ sequencing primer pMRB101-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cloning information may not be accurate. The plasmid was used for transfection by the Omary lab. For example, see Ku et al 2002 JBC 277:10775, Ku et al 1998 EMBO 17:1892.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
human K8 was a gift from M Bishr Omary (Addgene plasmid # 14742 ; http://n2t.net/addgene:14742 ; RRID:Addgene_14742) -
For your References section:
Keratin 8 phosphorylation by p38 kinase regulates cellular keratin filament reorganization: modulation by a keratin 1-like disease causing mutation. Ku NO, Azhar S, Omary MB. J Biol Chem. 2002 Mar 29. 277(13):10775-82. 10.1074/jbc.M107623200 PubMed 11788583