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Addgene

pT7-EGFP-C1-HsNot1iso1-P1209YP1257Y_R
(Plasmid #147374)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 147374 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Unknown
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HsNot1iso1-P1209YP1257Y
  • Species
    H. sapiens (human)
  • Mutation
    A deletion of 5 AA 822-827 SKMKPS-> T and one silent mutation compared to the sequence of isoform 1 given by NCBI: NM_016284.4
  • Entrez Gene
    CNOT1 (a.k.a. AD-005, CDC39, HPE12, NOT1, NOT1H)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT7-EGFP-C1-HsNot1iso1-P1209YP1257Y_R was a gift from Elisa Izaurralde (Addgene plasmid # 147374 ; http://n2t.net/addgene:147374 ; RRID:Addgene_147374)

  • For your References section:

    The structural basis for the interaction between the CAF1 nuclease and the NOT1 scaffold of the human CCR4-NOT deadenylase complex. Petit AP, Wohlbold L, Bawankar P, Huntzinger E, Schmidt S, Izaurralde E, Weichenrieder O. Nucleic Acids Res. 2012 Nov;40(21):11058-72. doi: 10.1093/nar/gks883. Epub 2012 Sep 12. 10.1093/nar/gks883 PubMed 22977175
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