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Addgene

pCGN R-Ras 38V, CVLS
(Plasmid #14735)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14735 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCGN
  • Backbone size w/o insert (bp) 7500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    R-ras
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    680
  • Mutation
    38V, CVLS
  • Entrez Gene
    RRAS (a.k.a. R-Ras)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCGN R-Ras 38V, CVLS was a gift from Adrienne Cox (Addgene plasmid # 14735 ; http://n2t.net/addgene:14735 ; RRID:Addgene_14735)

  • For your References section:

    Mammalian expression vectors for Ras family proteins: generation and use of expression constructs to analyze Ras family function. Fiordalisi JJ, Johnson RL, Ulku AS, Der CJ, Cox AD. Methods Enzymol. 2001 . 332():3-36. PubMed 11305105
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