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pAc5.1B-EGFP-DmNot4-dsRNAres_Q
(Plasmid #147339)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 147339 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Unknown
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    DmNot4-dsRNAres
  • Species
    D. melanogaster (fly)
  • Mutation
    7 silent mutations and 5 non silent mutations G207S, S402G, A405T, V537D, I899M and a deletion of 3 AA compared to the sequence given by NCBI:NM_164919.2; the annotated variations were found in two different clones from 2 independent experiments.
  • Entrez Gene
    CG31716 (a.k.a. CG5244, cg5251, CG5251, Dmel\CG31716, not3/5, not4, NOT4)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAc5.1B-EGFP-DmNot4-dsRNAres_Q was a gift from Elisa Izaurralde (Addgene plasmid # 147339 ; http://n2t.net/addgene:147339 ; RRID:Addgene_147339)

  • For your References section:

    A conserved CAF40-binding motif in metazoan NOT4 mediates association with the CCR4-NOT complex. Keskeny C, Raisch T, Sgromo A, Igreja C, Bhandari D, Weichenrieder O, Izaurralde E. Genes Dev. 2019 Feb 1;33(3-4):236-252. doi: 10.1101/gad.320952.118. Epub 2019 Jan 28. 10.1101/gad.320952.118 PubMed 30692204
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