pBABE H-Ras 61L, 186S
(Plasmid #14719)

• Depositing Lab: Adrienne Cox
• Publication: Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

Backbone

• Vector backbone: pBABE puro
• Backbone size w/o insert (bp): 5169
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: H-ras
• Species: H. sapiens (human)
• Insert Size (bp): 580
• Mutation: 61L, 186S
• Entrez Gene: HRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: pBABE 3'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Channing Der

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Prenylation mutant: does not undergo farnesylation or any other CAAX-signaled posttranslational modification, including palmitoylation; cytosolic, nontransforming; dominant inhibitory for activated Ras, by competition for effectors.