Lentiviral-TOP-dGFP-reporter
(Plasmid #14715)

• Purpose: Beta-catenin reporter - 3rd generation self-inactivating lentiviral plasmid LEF-1/TCF-responsive promoter driven d2-eGFP (destabilized, half-life of 2h).
• Depositing Lab: Tannishtha Reya
• Publication: Reya et al Nature. 2003 May 22. 423(6938):409-14.

Backbone

• Vector backbone: pRRL.sin-18.ppt
• Backbone size w/o insert (bp): 7000
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LEF-1/TCF-responsive promoter
• Alt name: beta catenin reporter
• Insert Size (bp): 100
• Entrez Gene: Ctnnb1 (a.k.a. Bfc, Catnb, Mesc)
• Tag / Fusion Protein:
  • d2 eGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

d2-eGFP gene (destabilized, half-life of 2 h; Clontech) was cloned downstream of a LEF-1/TCF-responsive promoter, containing three LEF-1/TCF binding motifs and a TATA box. This cassette was then cloned into a self-inactivating lentiviral vector plasmid.