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MSCV-dn-XSu(H)-IRES-GFP
(Plasmid #14714)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14714 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    MSCV-IRES-GFP
  • Backbone size w/o insert (bp) 6573
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dn XSu
  • Alt name
    XSu(H)
  • Species
    X. laevis (frog)
  • Entrez Gene
    rbpj (a.k.a. CBF1, RBPSUH, Su(H), XSu(H), xsu(h)1)
  • Tag / Fusion Protein
    • IRES-GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer N/A
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    dnXSu(H) is from Dr. Chris Kintner.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For producing retroviruses that express a dominant negative mutant form of xenopus suppressor of hairless (dnXSu(H); the xenopus homolog of CBF-1)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV-dn-XSu(H)-IRES-GFP was a gift from Tannishtha Reya (Addgene plasmid # 14714)

  • For your References section:

    Integration of Notch and Wnt signaling in hematopoietic stem cell maintenance. Duncan AW, Rattis FM, DiMascio LN, Congdon KL, Pazianos G, Zhao C, Yoon K, Cook JM, Willert K, Gaiano N, Reya T. Nat Immunol. 2005 Mar . 6(3):314-22. 10.1038/ni1164 PubMed 15665828
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