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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 14712 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneEMSV
- Backbone size w/o insert (bp) 5300
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemyogenin
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SpeciesR. norvegicus (rat)
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Insert Size (bp)1700
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Entrez GeneMyog
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
Resource Information
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EMSV-myogenin (puro) was a gift from Michael Rudnicki (Addgene plasmid # 14712 ; http://n2t.net/addgene:14712 ; RRID:Addgene_14712) -
For your References section:
The molecular regulation of myogenesis. Sabourin LA, Rudnicki MA. Clin Genet. 2000 Jan . 57(1):16-25. 10.1034/j.1399-0004.2000.570103.x PubMed 10733231