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pAc5.1B-EGFP-DmGW182_604-1115_N
(Plasmid #147060)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 147060 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Unknown
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    DmGW182_604-1115
  • Species
    D. melanogaster (fly)
  • Mutation
    Two non silent mutations V704A and N799S and one silent mutation G2295A compared to the sequence given by NM_166780 varA.
  • Entrez Gene
    gw (a.k.a. Dmel_CG31992, CG11484, CG31992, CG9905, Dmel\CG31992, GQ182, GW, GW182, Gw, Gw182, anon-EST:Posey205, dGW182, gw182)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAc5.1B-EGFP-DmGW182_604-1115_N was a gift from Elisa Izaurralde (Addgene plasmid # 147060 ; http://n2t.net/addgene:147060 ; RRID:Addgene_147060)

  • For your References section:

    The interactions of GW182 proteins with PABP and deadenylases are required for both translational repression and degradation of miRNA targets. Huntzinger E, Kuzuoglu-Ozturk D, Braun JE, Eulalio A, Wohlbold L, Izaurralde E. Nucleic Acids Res. 2013 Jan;41(2):978-94. doi: 10.1093/nar/gks1078. Epub 2012 Nov 21. 10.1093/nar/gks1078 PubMed 23172285
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