pAc5.1B-lambdaN-HA-DmBruno_N
(Plasmid #147030)

• Purpose: Insect Expression of DmBruno
• Depositing Lab: Elisa Izaurralde
• Publication: Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. doi: 10.1101/gad.17136311.

Backbone

• Vector backbone: Unknown
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: DmBruno
• Alt name: aret, arrest
• Alt name: bruno, bru CG31762
• Species: D. melanogaster (fly)
• Mutation: three non silent mutations S88T, H203R and A449R compared to the sequence given by NCBI: NM_165004.2
• Entrez Gene: aret (a.k.a. Dmel_CG31762, ARET, Aret, BcDNA:GM15173, Bru, Bruno, CG31762, CG6319, Dm Bru, Dmel\CG31762, NEST:bs01g04, NEST:bs34h11, aret/bruno1, arrest/Bruno, bru, bruno, ms(2)01284)

Cloning Information

• Cloning method: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact before placing your order to request that our lab fully sequence the plasmid.