pcDNA3 Flag beta-1-adrenergic-receptor
(Plasmid #14698)

• Depositing Lab: Robert Lefkowitz
• Publication: Tang et al Proc Natl Acad Sci U S A. 1999 Oct 26. 96(22):12559-64.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: B1AR
• Alt name: beta-1 adrenergic receptor
• Species: H. sapiens (human)
• Insert Size (bp): 1600
• GenBank ID: P08588.2
• Entrez Gene: ADRB1 (a.k.a. ADRB1R, B1AR, BETA1AR, FNSS2, RHR)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene Sanger sequencing found G389R in the B1AR translation