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Addgene

pcDNA3 barr2 HA
(Plasmid #14692)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14692 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    beta-arrestin 2
  • Alt name
    arrestin 3
  • Alt name
    arrb2
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1200
  • GenBank ID
    NM_012911
  • Entrez Gene
    Arrb2 (a.k.a. BARRES)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 barr2 HA was a gift from Robert Lefkowitz (Addgene plasmid # 14692 ; http://n2t.net/addgene:14692 ; RRID:Addgene_14692)

  • For your References section:

    Beta-arrestin-dependent formation of beta2 adrenergic receptor-Src protein kinase complexes. Luttrell LM, Ferguson SS, Daaka Y, Miller WE, Maudsley S, Della Rocca GJ, Lin F, Kawakatsu H, Owada K, Luttrell DK, Caron MG, Lefkowitz RJ. Science. 1999 Jan 29. 283(5402):655-61. 10.1126/science.283.5402.655 PubMed 9924018
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