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Addgene

pRK5 bovine GRK2
(Plasmid #14691)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14691 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GRK2
  • Alt name
    beta adrenergic receptor kinase 1
  • Alt name
    BARK1
  • Alt name
    ADRBK1
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
    2300
  • Entrez Gene
    GRK2 (a.k.a. ADRBK1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer Sp6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Using polymerase chain reaction, a 5'

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5 bovine GRK2 was a gift from Robert Lefkowitz (Addgene plasmid # 14691 ; http://n2t.net/addgene:14691 ; RRID:Addgene_14691)

  • For your References section:

    Feedback inhibition of G protein-coupled receptor kinase 2 (GRK2) activity by extracellular signal-regulated kinases. Pitcher JA, Tesmer JJ, Freeman JL, Capel WD, Stone WC, Lefkowitz RJ. J Biol Chem. 1999 Dec 3. 274(49):34531-4. 10.1074/jbc.274.49.34531 PubMed 10574913
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