pT7-EGFP-C1-HsNot7-D40AE42A_L
(Plasmid #146890)

• Purpose: Mammalian Expression of HsNot7-D40AE42A. Please note that this plasmid does not contain the T7 promoter.
• Depositing Lab: Elisa Izaurralde
• Publication: Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94. doi: 10.1093/nar/gks1078. Epub 2012 Nov 21.

Backbone

• Vector backbone: Unknown
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HsNot7-D40AE42A
• Alt name: CAF1
• Species: H. sapiens (human)
• Entrez Gene: CNOT7 (a.k.a. CAF-1, CAF1, Caf1a, hCAF-1)

Cloning Information

• Cloning method: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

sequence extracted from NCBI: NM_013354.5 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact before placing your order to request that our lab fully sequence the plasmid.