PECAM-H2B-GFP-Hygro
(Plasmid #14684)

• Depositing Lab: Victoria Bautch
• Publication: Zeng et al Blood. 2007 Feb 15. 109(4):1345-52.

Backbone

• Vector backbone: EGFP-1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 6200
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PECAM promoter/enhancer
• Alt name: PECAM
• Species: M. musculus (mouse)
• Insert Size (bp): 8000
• Entrez Gene: Pecam1 (a.k.a. Cd31, PECAM-1, Pecam)
• Tags / Fusion Proteins:
  • PGK-Hygro (N terminal on backbone)
  • H2B-GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: EGFP-N

Resource Information

• A portion of this plasmid was derived from a plasmid made by: H. Scott Baldwin, Vanderbilt

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry