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Addgene

pPROK AdoHcyase
(Plasmid #14683)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14683 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPROK-1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4600
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    adenosyl-homocysteine hydrolase
  • Alt name
    AdoHcyase
  • Alt name
    AHCY
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1300
  • Entrez Gene
    AHCY (a.k.a. SAHH, adoHcyase)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer tac promoter
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The AdoHcyase coding sequence was derived from human placenta AdoHcyase cDNA clone 20-1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPROK AdoHcyase was a gift from Michael Hershfield (Addgene plasmid # 14683 ; http://n2t.net/addgene:14683 ; RRID:Addgene_14683)

  • For your References section:

    Sequence of full length cDNA for human S-adenosylhomocysteine hydrolase. Coulter-Karis DE, Hershfield MS. Ann Hum Genet. 1989 May . 53(Pt 2):169-75. 10.1111/j.1469-1809.1989.tb01781.x PubMed 2596825
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