pT7-EGFP-C1-HsXRN1_1-1173_K
(Plasmid
#146785)
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PurposeMammalian Expression of HsXRN1_1-1173
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 146785 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneUnknown
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHsXRN1_1-1173
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SpeciesH. sapiens (human)
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Mutationone silent mutation compared to the sequence given by NCBI : NM_019001.3.
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Entrez GeneXRN1 (a.k.a. SEP1)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pT7-EGFP-C1-HsXRN1_1-1173_K was a gift from Elisa Izaurralde (Addgene plasmid # 146785 ; http://n2t.net/addgene:146785 ; RRID:Addgene_146785) -
For your References section:
A direct interaction between DCP1 and XRN1 couples mRNA decapping to 5' exonucleolytic degradation. Braun JE, Truffault V, Boland A, Huntzinger E, Chang CT, Haas G, Weichenrieder O, Coles M, Izaurralde E. Nat Struct Mol Biol. 2012 Dec;19(12):1324-31. doi: 10.1038/nsmb.2413. Epub 2012 Nov 11. 10.1038/nsmb.2413 PubMed 23142987