pT7-EGFP-C1-HsNot11_K
(Plasmid
#146776)
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PurposeMammalian Expression of HsNot11
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 146776 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneUnknown
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHsNot11
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Alt nameC2ORF29
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SpeciesH. sapiens (human)
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Entrez GeneCNOT11 (a.k.a. C2orf29, C40)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
ORF extracted from NCBI: NM_017546.4 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pT7-EGFP-C1-HsNot11_K was a gift from Elisa Izaurralde (Addgene plasmid # 146776 ; http://n2t.net/addgene:146776 ; RRID:Addgene_146776) -
For your References section:
GW182 proteins directly recruit cytoplasmic deadenylase complexes to miRNA targets. Braun JE, Huntzinger E, Fauser M, Izaurralde E. Mol Cell. 2011 Oct 7;44(1):120-33. 10.1016/j.molcel.2011.09.007 PubMed 21981923