pT7-EGFP-C1-HsNot10_K
(Plasmid #146775)

• Purpose: Mammalian Expression of HsNot10
• Depositing Lab: Elisa Izaurralde
• Publication: Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Backbone

• Vector backbone: Unknown
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HsNot10
• Alt name: CNOT10 cloned from Kazusa clone: on15275
• Species: H. sapiens (human)
• Mutation: one silent mutation compared to the sequence given by NCBI: NM_015442.1
• Entrez Gene: CNOT10 (a.k.a. FLJ12890, FLJ13165, DKFZp434K115)

Cloning Information

• Cloning method: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact before placing your order to request that our lab fully sequence the plasmid.