Syn3D146
(Plasmid
#14677)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14677 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePCDNA4/TO/myc-His
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5150
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Vector typeMammalian Expression
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSyntaxin3
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Alt nameSTX3
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SpeciesH. sapiens (human)
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MutationInsert is cloned into a modified vector; aa 1-146 deleted
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Entrez GeneSTX3 (a.k.a. DIAR12, MVID2, RDMVID, STX3A)
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Tag
/ Fusion Protein
- 2xMyc-His (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMVF
- 3′ sequencing primer pcDNA3.1/BGH reverse primier (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Syn3D146 was a gift from Thomas Weimbs (Addgene plasmid # 14677 ; http://n2t.net/addgene:14677 ; RRID:Addgene_14677) -
For your References section:
Apical targeting of syntaxin 3 is essential for epithelial cell polarity. Sharma N, Low SH, Misra S, Pallavi B, Weimbs T. J Cell Biol. 2006 Jun 19. 173(6):937-48. 10.1083/jcb.200603132 PubMed 16785322