pRc/RSV Flag MKK3(glu)
(Plasmid #14670)

• Depositing Lab: Roger Davis
• Publication: Raingeaud et al Mol Cell Biol. 1996 Mar . 16(3):1247-55.

Backbone

• Vector backbone: pRc/RSV
• Backbone size w/o insert (bp): 5224
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MKK3(glu)
• Alt name: MKK3
• Species: H. sapiens (human)
• Insert Size (bp): 1006
• Mutation: Ser-189Glu Thr-193Glu
• Entrez Gene: MAP2K3 (a.k.a. MAPKK3, MEK3, MKK3, PRKMK3, SAPKK-2, SAPKK2)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hind III (not destroyed)
• 3′ cloning site: Spe1 (not destroyed)
• 5′ sequencing primer: pREP-fwd (GCTCGATACAATAAACGCC )
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry