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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14669 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRc/RSV
- Backbone size w/o insert (bp) 5224
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMKK3(ala)
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Alt nameMKK3
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SpeciesH. sapiens (human)
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Insert Size (bp)1005
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MutationSer-189Ala Thr-193Ala
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Entrez GeneMAP2K3 (a.k.a. MAPKK3, MEK3, MKK3, PRKMK3, SAPKK-2, SAPKK2)
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Hind III (not destroyed)
- 3′ cloning site Spe1 (not destroyed)
- 5′ sequencing primer pREP-fwd (GCTCGATACAATAAACGCC )
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRc/RSV Flag MKK3(ala) was a gift from Roger Davis (Addgene plasmid # 14669 ; http://n2t.net/addgene:14669 ; RRID:Addgene_14669) -
For your References section:
MKK3- and MKK6-regulated gene expression is mediated by the p38 mitogen-activated protein kinase signal transduction pathway. Raingeaud J, Whitmarsh AJ, Barrett T, Derijard B, Davis RJ. Mol Cell Biol. 1996 Mar . 16(3):1247-55. PubMed 8622669