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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14667 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepQE9
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Backbone manufacturerQiagen
- Backbone size w/o insert (bp) 3300
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Growth instructionsNeeds strain with lacIq
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namep97
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Alt nameVCP
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Alt namevalosin-containing protein
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SpeciesM. musculus (mouse)
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Insert Size (bp)2415
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MutationE305/578Q
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GenBank IDNM_009503
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Entrez GeneVcp (a.k.a. 3110001E05, CDC48, p97, p97/VCP)
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Tag
/ Fusion Protein
- RGS6xHis (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pQE9-His-p97(QQ) was a gift from Graham Warren (Addgene plasmid # 14667 ; http://n2t.net/addgene:14667 ; RRID:Addgene_14667) -
For your References section:
The AAA ATPase Cdc48/p97 and its partners transport proteins from the ER into the cytosol. Ye Y, Meyer HH, Rapoport TA. Nature. 2001 Dec 6. 414(6864):652-6. 10.1038/414652a PubMed 11740563