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pQE9-His-p97(wt)
(Plasmid #14666)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14666 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQE9
  • Backbone manufacturer
    Qiagen
  • Backbone size w/o insert (bp) 3300
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Growth instructions
    Needs strain with lacIq
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    p97
  • Alt name
    VCP
  • Alt name
    valosin-containing protein
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2415
  • Mutation
    See depositor comments below.
  • GenBank ID
    NM_009503
  • Entrez Gene
    Vcp (a.k.a. 3110001E05, CDC48, p97, p97/VCP)
  • Tag / Fusion Protein
    • RGS6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHi (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS results identified a V206 variant in p97 that matches NCBI reference CAA78412.1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQE9-His-p97(wt) was a gift from Graham Warren (Addgene plasmid # 14666 ; http://n2t.net/addgene:14666 ; RRID:Addgene_14666)

  • For your References section:

    A complex of mammalian ufd1 and npl4 links the AAA-ATPase, p97, to ubiquitin and nuclear transport pathways. Meyer HH, Shorter JG, Seemann J, Pappin D, Warren G. EMBO J. 2000 May 15. 19(10):2181-92. 10.1093/emboj/19.10.2181 PubMed 10811609
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