pACT2 V1H (P#663)
(Plasmid
#14665)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14665 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepACT2
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 7500
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameV1H
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Alt nameV-ATPase subunit H
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SpeciesH. sapiens (human)
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Entrez GeneATP6V1H (a.k.a. CGI-11, MSTP042, NBP1, SFD, SFDalpha, SFDbeta, VMA13)
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Tag
/ Fusion Protein
- Gal4 AD (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer Gal4 AD (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For two-hybrid assay.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pACT2 V1H (P#663) was a gift from Matija Peterlin (Addgene plasmid # 14665 ; http://n2t.net/addgene:14665 ; RRID:Addgene_14665) -
For your References section:
Subunit H of the V-ATPase involved in endocytosis shows homology to beta-adaptins. Geyer M, Fackler OT, Peterlin BM. Mol Biol Cell. 2002 Jun . 13(6):2045-56. 10.1091/mbc.02-02-0026 PubMed 12058068