Skip to main content
Addgene

pACT2 V1H (P#663)
(Plasmid #14665)

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14665 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pACT2
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 7500
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    V1H
  • Alt name
    V-ATPase subunit H
  • Species
    H. sapiens (human)
  • Entrez Gene
    ATP6V1H (a.k.a. CGI-11, MSTP042, NBP1, SFD, SFDalpha, SFDbeta, VMA13)
  • Tag / Fusion Protein
    • Gal4 AD (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer Gal4 AD
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For two-hybrid assay.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACT2 V1H (P#663) was a gift from Matija Peterlin (Addgene plasmid # 14665 ; http://n2t.net/addgene:14665 ; RRID:Addgene_14665)
  • For your References section:

    Subunit H of the V-ATPase involved in endocytosis shows homology to beta-adaptins. Geyer M, Fackler OT, Peterlin BM. Mol Biol Cell. 2002 Jun . 13(6):2045-56. 10.1091/mbc.02-02-0026 PubMed 12058068