pMT-NTAP-DmCCR4_J
(Plasmid #146649)

• Purpose: Insect Expression of DmCCR4
• Depositing Lab: Elisa Izaurralde
• Publication: Izaurralde lab plasmids (unpublished)

Backbone

• Vector backbone: pMK33-NTAP
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: DmCCR4
• Alt name: twin
• Alt name: CG31137
• Species: D. melanogaster (fly)
• Mutation: 4 silent mutations compared to the sequence given by NCBI: NM_170129.2
• Entrez Gene: twin (a.k.a. Dmel_CG31137, BEST:GH20274, CCR4, CG17741, CG31137, CG5534, CT39331, Ccr4, Dmel\CG31137, Twin, ccr4, twn)

Cloning Information

• Cloning method: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pMK33-NTAP and pMK33-CTAP vectors are designed for making proteinfusions tagged with the TAP tag and for generating stableDrosophila cell lines under Hygromycin selection. TAP fusions areinducible with copper sulfate. The parental vector, pMK33 (pMt/Hy),was created by Michael Koelle. Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact before placing your order to request that our lab fully sequence the plasmid.