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Addgene

pGal CycK (P#844)
(Plasmid #14664)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14664 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSG424
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CycK
  • Alt name
    cyclin K
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1000
  • Entrez Gene
    CCNK (a.k.a. CPR4, IDDHDF)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid pGal (pSG424) contains the coding sequence of GAL4-(1-147) immediately followed by a multiple cloning site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGal CycK (P#844) was a gift from Matija Peterlin (Addgene plasmid # 14664 ; http://n2t.net/addgene:14664 ; RRID:Addgene_14664)

  • For your References section:

    P-TEFb containing cyclin K and Cdk9 can activate transcription via RNA. Lin X, Taube R, Fujinaga K, Peterlin BM. J Biol Chem. 2002 May 10. 277(19):16873-8. 10.1074/jbc.M200117200 PubMed 11884399
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