pG6(5'Pro) (P#1477)
(Plasmid
#14659)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14659 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneCAT reporter
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameUAS repeats
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains HIV LTR linked to the CAT reporter gene. Five repeats of the synthetic Gal4-binding sites (UAS) were introduced upstream of the three Sp1 sites. These UAS repeats replaced the enhancer (EN) in the HIV LTR. The promoter contains the CP, i.e., the TATA box and INR sequences.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pG6(5'Pro) (P#1477) was a gift from Matija Peterlin (Addgene plasmid # 14659 ; http://n2t.net/addgene:14659 ; RRID:Addgene_14659) -
For your References section:
Interaction between P-TEFb and the C-terminal domain of RNA polymerase II activates transcriptional elongation from sites upstream or downstream of target genes. Taube R, Lin X, Irwin D, Fujinaga K, Peterlin BM. Mol Cell Biol. 2002 Jan . 22(1):321-31. 10.1128/MCB.22.1.321-331.2002 PubMed 11739744