pT7-EGFP-C1-HseIF4A3_I
(Plasmid
#146581)
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PurposeMammalian Expression of HseIF4A3
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 146581 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneUnknown
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHseIF4A3
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SpeciesH. sapiens (human)
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Entrez GeneEIF4A3 (a.k.a. DDX48, Fal1, MUK34, NMP265, NUK34, RCPS, eIF-4A-III, eIF4A-III, eIF4AIII)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
ORF extracted from NCBI:NM_014740.3 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pT7-EGFP-C1-HseIF4A3_I was a gift from Elisa Izaurralde (Addgene plasmid # 146581 ; http://n2t.net/addgene:146581 ; RRID:Addgene_146581) -
For your References section:
An eIF4AIII-containing complex required for mRNA localization and nonsense-mediated mRNA decay. Palacios IM, Gatfield D, St Johnston D, Izaurralde E. Nature. 2004 Feb 19. 427(6976):753-7. 10.1038/nature02351 PubMed 14973490