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Addgene

pEF NEF NBP1 (P#375)
(Plasmid #14657)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14657 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF-BOS
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NEF NBP1
  • Alt name
    Nef
  • Alt name
    Nef binding protein 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2100
  • Entrez Gene
    ATP6V1H (a.k.a. CGI-11, MSTP042, NBP1, SFD, SFDalpha, SFDbeta, VMA13)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer EF1a forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HA tag between Nef and NBP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF NEF NBP1 (P#375) was a gift from Matija Peterlin (Addgene plasmid # 14657 ; http://n2t.net/addgene:14657 ; RRID:Addgene_14657)

  • For your References section:

    Interactions between HIV1 Nef and vacuolar ATPase facilitate the internalization of CD4. Lu X, Yu H, Liu SH, Brodsky FM, Peterlin BM. Immunity. 1998 May . 8(5):647-56. 10.1016/S1074-7613(00)80569-5 PubMed 9620685
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