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Addgene

pcDNAI Tat C30G HA (P#38)
(Plasmid #14656)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14656 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNAI/Amp
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tat
  • Species
    HIV
  • Insert Size (bp)
    1080
  • Mutation
    C30G C34G
  • Entrez Gene
    tat (a.k.a. HIV1gp5)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mutant Tat gene: C30G is in the activation domain of Tat and renders Tat inactive without affecting its RNA-binding ability or protein expression levels.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNAI Tat C30G HA (P#38) was a gift from Matija Peterlin (Addgene plasmid # 14656 ; http://n2t.net/addgene:14656 ; RRID:Addgene_14656)

  • For your References section:

    The human immunodeficiency virus transactivator Tat interacts with the RNA polymerase II holoenzyme. Cujec TP, Cho H, Maldonado E, Meyer J, Reinberg D, Peterlin BM. Mol Cell Biol. 1997 Apr . 17(4):1817-23. PubMed 9121429
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