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pCIneo-V5-SBP-HsSmg6-del817-1238-siRNAres_I
(Plasmid #146555)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 146555 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI-neo
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HsSmg6-del817-1238-siRNAres
  • Species
    H. sapiens (human)
  • Mutation
    two silent mutations T594C, A699G and two non silent mutation G872C (R291P), A1022C (N341T) compared to the sequence given by NCBI: NM_017575.4
  • Entrez Gene
    SMG6 (a.k.a. C17orf31, EST1A, SMG-6, hSMG5/7a)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCIneo-V5-SBP-HsSmg6-del817-1238-siRNAres_I was a gift from Elisa Izaurralde (Addgene plasmid # 146555 ; http://n2t.net/addgene:146555 ; RRID:Addgene_146555)

  • For your References section:

    SMG6 interacts with the exon junction complex via two conserved EJC-binding motifs (EBMs) required for nonsense-mediated mRNA decay. Kashima I, Jonas S, Jayachandran U, Buchwald G, Conti E, Lupas AN, Izaurralde E. Genes Dev. 2010 Nov 1. 24(21):2440-50. 10.1101/gad.604610 PubMed 20930030
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