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pLINK bTat (P#697)
(Plasmid #14653)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14653 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    EF-pLINK2
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    bTat
  • Species
    BIV
  • Insert Size (bp)
    309
  • Mutation
    Amino acids 1-103
  • Tag / Fusion Protein
    • myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer Myc
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

bTat was amplified by PCR from BIV127 provirus and the fragment was subcloned into NcoI-EcoRI-cleaved modified pEFBOS in frame with an N-terminal Myc-epitope tag (pbTat).

BIV Tat contains R10M, P52L, I61V and S65P compared to closest reference sequence (AAA42775.1).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLINK bTat (P#697) was a gift from Matija Peterlin (Addgene plasmid # 14653 ; http://n2t.net/addgene:14653 ; RRID:Addgene_14653)

  • For your References section:

    Binding of Tat to TAR and recruitment of positive transcription elongation factor b occur independently in bovine immunodeficiency virus. Barboric M, Taube R, Nekrep N, Fujinaga K, Peterlin BM. J Virol. 2000 Jul . 74(13):6039-44. 10.1128/JVI.74.13.6039-6044.2000 PubMed 10846086
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