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Addgene

pcDNA3 CDK8 HA (P#634)
(Plasmid #14649)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14649 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cdk8
  • Species
    H. sapiens (human)
  • Entrez Gene
    CDK8 (a.k.a. IDDHBA, K35)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 CDK8 HA (P#634) was a gift from Matija Peterlin (Addgene plasmid # 14649 ; http://n2t.net/addgene:14649 ; RRID:Addgene_14649)

  • For your References section:

    c-Myc recruits P-TEFb for transcription, cellular proliferation and apoptosis. Kanazawa S, Soucek L, Evan G, Okamoto T, Peterlin BM. Oncogene. 2003 Aug 28. 22(36):5707-11. 10.1038/sj.onc.1206800 PubMed 12944920
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