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Addgene

Mouse ZP3 (JD#47)
(Plasmid #14646)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14646 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript KS
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2961
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ZP3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1400
  • Entrez Gene
    Zp3 (a.k.a. Zp-, Zp-3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (not destroyed)
  • 3′ cloning site EcoR I (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mouse ZP3 (JD#47) was a gift from Jurrien Dean (Addgene plasmid # 14646 ; http://n2t.net/addgene:14646 ; RRID:Addgene_14646)

  • For your References section:

    Molecular analysis of cDNA coding for ZP3, a sperm binding protein of the mouse zona pellucida. Ringuette MJ, Chamberlin ME, Baur AW, Sobieski DA, Dean J. Dev Biol. 1988 Jun . 127(2):287-95. 10.1016/0012-1606(88)90315-6 PubMed 3378665
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