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Addgene

Mouse FIGalpha (JD#435)
(Plasmid #14643)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14643 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript KS
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2961
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FIG alpha
  • Alt name
    Factor In the Germline
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    730

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mouse FIGalpha (JD#435) was a gift from Jurrien Dean (Addgene plasmid # 14643 ; http://n2t.net/addgene:14643 ; RRID:Addgene_14643)

  • For your References section:

    FIGalpha, a germ cell specific transcription factor involved in the coordinate expression of the zona pellucida genes. Liang L, Soyal SM, Dean J. Development. 1997 Dec . 124(24):4939-47. PubMed 9362457
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