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Addgene

pAc5.1B-lambdaN-HA-DmNot4_F
(Plasmid #146275)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 146275 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Unknown
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    DmNot4
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    CG31716 (a.k.a. CG5244, cg5251, CG5251, Dmel\CG31716, not3/5, not4, NOT4)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

sequence given by NCBI:NM_001273415.1; Uniprot.: M9PCL9 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAc5.1B-lambdaN-HA-DmNot4_F was a gift from Elisa Izaurralde (Addgene plasmid # 146275 ; http://n2t.net/addgene:146275 ; RRID:Addgene_146275)

  • For your References section:

    The silencing domain of GW182 interacts with PABPC1 to promote translational repression and degradation of miRNA targets and is required for target release. Zekri L, Huntzinger E, Heimstädt S, Izaurralde E. Mol Cell Biol. 2009 Sep 21. ():. 10.1128/MCB.01081-09 PubMed 19797087
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