pEF myc Gal cyclin T1 (1-726) (P#1026)
(Plasmid #14626)

• Depositing Lab: Matija Peterlin
• Publication: Taube et al Mol Cell Biol. 2002 Jan . 22(1):321-31.

Backbone

• Vector backbone: pEF-BOS-myc
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: cyclin T1
• Alt name: cyclinT1
• Alt name: cycT1
• Species: H. sapiens (human)
• Insert Size (bp): 2186
• Entrez Gene: CCNT1 (a.k.a. CCNT, CYCT1, HIVE1)
• Tags / Fusion Proteins:
  • myc (N terminal on backbone)
  • Gal (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: EF1a forward primer

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Full-length cyclin T1 fused to the DBD of Gal4(1–147) and inserted at the BamHI/XbaI sites of pEF-Bos-myc(EF). The cyclin insert contains H519R, S566P, P712S, and L724F compared to closest cyclin T1 reference sequence NP_001231.2.