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Addgene

pBS mCD4
(Plasmid #14613)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14613 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CD4
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Cd4 (a.k.a. L3T4, Ly-4)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer M13R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS mCD4 was a gift from Dan Littman (Addgene plasmid # 14613 ; http://n2t.net/addgene:14613 ; RRID:Addgene_14613)

  • For your References section:

    The envelope glycoprotein of the human immunodeficiency virus binds to the immunoglobulin-like domain of CD4. Landau NR, Warton M, Littman DR. Nature. 1988 Jul 14. 334(6178):159-62. 10.1038/334159a0 PubMed 3260352
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