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Addgene

pGEX-2TK cyclin T1 (1-300) (P#432)
(Plasmid #14608)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14608 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX 2TK
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cyclin T1 (1-300)
  • Alt name
    cyclinT1
  • Alt name
    cycT1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    900
  • Mutation
    amino acids 1-300
  • Entrez Gene
    CCNT1 (a.k.a. CCNT, CYCT1, HIVE1)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pGEX 5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene's quality control sequence shows a K106R mutation in cyclin T1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-2TK cyclin T1 (1-300) (P#432) was a gift from Matija Peterlin (Addgene plasmid # 14608 ; http://n2t.net/addgene:14608 ; RRID:Addgene_14608)

  • For your References section:

    Interactions between human cyclin T, Tat, and the transactivation response element (TAR) are disrupted by a cysteine to tyrosine substitution found in mouse cyclin T. Fujinaga K, Taube R, Wimmer J, Cujec TP, Peterlin BM. Proc Natl Acad Sci U S A. 1999 Feb 16. 96(4):1285-90. 10.1073/pnas.96.4.1285 PubMed 9990016
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