pGEX-2TK cyclin T1 (P#468)
(Plasmid #14607)

• Depositing Lab: Matija Peterlin
• Publication: Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 96(4):1285-90.

Backbone

• Vector backbone: pGEX 2TK
• Backbone manufacturer: Amersham
• Backbone size w/o insert (bp): 5000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cyclin T1
• Alt name: cyclinT1
• Alt name: cycT1
• Species: H. sapiens (human)
• Insert Size (bp): 2186
• Entrez Gene: CCNT1 (a.k.a. CCNT, CYCT1, HIVE1)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pGEX-5
• 3′ sequencing primer: pGEX-3

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There is a H519R mutation in the ORF.