pGEX-2TK cyclin T1 (1-250) (P#467)
(Plasmid #14606)

• Depositing Lab: Matija Peterlin
• Publication: Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 96(4):1285-90.

Backbone

• Vector backbone: pGEX 2TK
• Backbone manufacturer: Amersham
• Backbone size w/o insert (bp): 5000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cyclin T1 (1-250)
• Alt name: cyclinT1
• Alt name: cycT1
• Species: H. sapiens (human)
• Insert Size (bp): 750
• Mutation: amino acids 1-250
• Entrez Gene: CCNT1 (a.k.a. CCNT, CYCT1, HIVE1)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pGEX 5'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene QC result indicates this construct contains amino acid residues 1 - 253.