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Addgene

pEF myc Gal cycT2b (P#1719)
(Plasmid #14600)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14600 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF-BOS-myc-Gal
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    cycT2
  • Alt name
    hCycT2
  • Alt name
    cyclin T2
  • Alt name
    cyclinT2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2193
  • Entrez Gene
    CCNT2 (a.k.a. CYCT2)
  • Tags / Fusion Proteins
    • myc (N terminal on backbone)
    • Gal (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer EF1a forward primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map for additional cloning information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF myc Gal cycT2b (P#1719) was a gift from Matija Peterlin (Addgene plasmid # 14600 ; http://n2t.net/addgene:14600 ; RRID:Addgene_14600)

  • For your References section:

    Transcriptional activity and substrate recognition of cyclin T2 from P-TEFb. Kurosu T, Zhang F, Peterlin BM. Gene. 2004 Dec 8. 343(1):173-9. 10.1016/j.gene.2004.08.027 PubMed 15563843
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