pCMV Flag cycT2b (P#1718)
(Plasmid #14599)

• Depositing Lab: Matija Peterlin
• Publication: Kurosu et al Gene. 2004 Dec 8. 343(1):173-9.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cycT2
• Alt name: hCycT2
• Alt name: cyclin T2
• Alt name: cyclinT2
• Species: H. sapiens (human)
• Insert Size (bp): 2193
• Mutation: A137T compared to NP_490595.1
• Entrez Gene: CCNT2 (a.k.a. CYCT2)
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not unique) (not destroyed)
• 3′ cloning site: ApaI (not destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

cycT2b contains an internal EcoRI site. See author's map for more information.