pEF myc cycT2b (P#1717)
(Plasmid
#14598)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14598 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEF-BOS-myc
- Backbone size w/o insert (bp) 5500
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namecycT2
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Alt namehCycT2
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Alt namecyclin T2
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Alt namecyclinT2
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SpeciesH. sapiens (human)
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Insert Size (bp)2193
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Entrez GeneCCNT2 (a.k.a. CYCT2)
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Tag
/ Fusion Protein
- myc (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site SpeI (not destroyed)
- 5′ sequencing primer EF1a forward primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See author's map for additional cloning information.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEF myc cycT2b (P#1717) was a gift from Matija Peterlin (Addgene plasmid # 14598 ; http://n2t.net/addgene:14598 ; RRID:Addgene_14598) -
For your References section:
Transcriptional activity and substrate recognition of cyclin T2 from P-TEFb. Kurosu T, Zhang F, Peterlin BM. Gene. 2004 Dec 8. 343(1):173-9. 10.1016/j.gene.2004.08.027 PubMed 15563843
Map uploaded by the depositor.
