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Addgene

pCMV Ub Lex HA (P#1707)
(Plasmid #14592)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14592 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5600
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LexA
  • Alt name
    Lex
  • Species
    E. coli
  • Insert Size (bp)
    834
  • Tags / Fusion Proteins
    • Ubiquitin (N terminal on insert)
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV Ub Lex HA (P#1707) was a gift from Matija Peterlin (Addgene plasmid # 14592 ; http://n2t.net/addgene:14592 ; RRID:Addgene_14592)

  • For your References section:

    VP16 and ubiquitin; binding of P-TEFb via its activation domain and ubiquitin facilitates elongation of transcription of target genes. Kurosu T, Peterlin BM. Curr Biol. 2004 Jun 22. 14(12):1112-6. 10.1016/j.cub.2004.06.020 PubMed 15203006
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